Dr. Holger Gärtner

The southern Mediterranean region, particularly North Africa, is a crucial area for biodiversity conservation. However, the impacts of climate change on plant species in this region are not well understood. Dendroecology, the study of tree rings, is a valuable technique for analyzing the effects of environmental changes on woody plants over time. In this study, we intend to assess the state of the art in dendrochronological research in North Africa and identify knowledge gaps and limitations in the field. The period of analysis spans from 1979 to 2023. We used all the available literature in Dendrobox and Google Scholar during this period. Our study revealed several research gaps in the region, including the need for more studies on the history of forest fires and their relationship to climate conditions in Morocco, Algeria, and Tunisia, the impact of climate on the anatomical characteristics of growth rings, and the effects of climate change on tree species diversity and forest health. Applying this technique in the future would allow for detailed insights into the effect of climate on the internal structure and growth of forest trees. The findings of this study will help guide future research and contribute to a better understanding of the climate-growth relationship of woody plants in North Africa.

Here we present a new workflow from taking increment cores in the field, storing and transporting them to the lab, to digitizing their tree rings for further analyses for subsequent dendroecological analyses. The procedure involves the use of new sample carriers for increment cores. These new Gärtner Schneider Core (GSC) holders are designed using three-dimensional (3D) modeling software and finally printed with a 3D printer. Using these mounts from the beginning in the field, the cores can directly be cut with a core microtome, and their surface can then be digitized without further rearrangement using a new high-resolution image-capturing system. They are thus available for direct analysis. This system allows digitizing tree rings from cores and disks, and also taking images from long micro sections (up to 40 cm) using transmitted light. This feature is of special interest for dendroecological and geomorphic applications to identify the onset of any disturbance in micro sections cut with a core-microtome.

In tropical and subtropical regions, much research is still required to explore the dendrochronological potential of their trees. This study aims to evaluate the anatomical structure and dendrochronological potential of three Mediterranean desert shrubs in Egypt (Lycium schweinfurthii var. schweinfurthii, L. europaeum, and Calligonum polygonoides subsp. comosum) supported by X-ray density. The results showed that the target species had distinct growth rings at macroscopic and microscopic levels. The vessel traits reflected the adaptability of each species with the prevailing arid climate conditions. After the exclusion of the non-correlated series, we obtained three site chronologies that cover the years 2013-2022 for L. schweinfurthii, 2012-2022 for L. europaeum, and 2011-2022 for C. comosum. The mean series intercorrelation was 0.746, 0.564, and 0.683 for L. schweinfurthii, L. europaeum, and C. comosum, respectively. The EPS (expressed population signal) values ranged from 0.72 to 0.80, while the SNR (species-to-noise ratio) ranged from 9.1 to 21.5. Compiling all series of L. schweinfurthii raised the EPS value to 0.86. The chronologies developed for the studied species were relatively short since we dealt with multi-stemmed shrubs. The average percentage difference between latewood density (LWD) and earlywood density (EWD) in C. comosum, L. europaeum, and L. schweinfurthii were 11.8% ± 5.5, 5.2%± 1.87, and 3.6% ± 1.86, respectively. X-ray densitometry helped in the precise determination of the ring borders of the studied species. The relationships between the radial growth of the studied species and the climate variables were weak to moderate but mostly not significant (i.e., r < 0.7). Generally, the radial growth of the target species had a weak to moderate positive correlation with temperature and precipitation during the wet season (winter), while negatively correlated with temperature for the rest of the year, particularly in summer. Our data agrees with earlier findings that ring formation starts at the beginning of the long vegetative stage, then the rest of the assimilated carbohydrates are directed to the flowering and fruiting at the end of the vegetative stages. For more efficient dendrochronological studies on subtropical and Mediterranean trees, we recommend carrying out xylogenesis studies, collection of phenological data, sampling 45-80 trees per species, using new techniques, and choosing homogeneous and close sites for wood sampling.

In dendroecological research, precise dating of each single growth ring is a basic requirement for all studies, focusing on ring-width variations only, chemical or isotope analyses, or wood anatomical studies. Independent of the sampling strategy for a certain study (e.g., climatology, geomorphology), the way samples are taken is crucial for their successful preparation and analyses.
Until recently, it was sufficient to use a (more or less) sharp increment corer to obtain core samples that could be sanded for further analyses. Since wood anatomical characteristics can be applied to long time series, the need to obtain high-quality increment cores has taken on a new meaning. Essentially, the corer needs to be sharp(ened) when used. When coring a tree by hand, there are some problems in handling the corer, resulting in the hidden occurrence of micro cracks along the entire core: When starting to drill by hand, the drill bit is strongly pressed against the bark and the outermost ring until the thread has fully entered the trunk. At the same time, the drill bit is moved up and down as well as sideward. Then, the corer is drilled all the way into the trunk; however, it is necessary to stop after each turn, change the grip, and turn again. All these movements, as well as the start/stop-coring, puts mechanical stress on the core. The resulting micro cracks make it impossible to create continuous micro sections, as they fall apart along all these cracks.
We present a protocol to overcome these obstacles by applying a new technique using a cordless drill to minimize these problems when coring a tree, as well as its effect on the preparation of long micro sections. This protocol includes the preparation of long micro sections, as well as a procedure to sharpen corers in the field.

Steile Schutthänge mit einer fehlenden Vegetationsdecke sind in den Alpen allgegenwärtig und führen in Kombination mit Niederschlägen nicht selten zu Erosion und flachgründigen Rutschungen. Um die Gefahr durch diese Massenbewegungen zu reduzieren, wird durch ingenieurbiologische Massnahmen eine flächendeckende Oberflächenstabilität angestrebt. Insbesondere die ersten drei egetationsperioden sind für den Erfolg solcher Massnahmen ausschlaggebend. Um den Pflanzen möglichst gut unter die Arme greifen zu können, wird der Einsatz von Mykorrhizapilzen im ingenieurbiologischen Hangverbau innerhalb eines Feldexperimentes im Arieschtobel (Kanton Graubünden) getestet. Im Mai 2010 wurden dazu Heckenlagen mit einer kommerziellen Pilzmischung geimpft und mit herkömmlichen Heckenlagen ohne Impfung sowie einer Kontrollfläche verglichen. Die Impfung führte zu höheren Überlebensraten der eingebrachten Pflanzen, zu einer höheren Bodenaggregatstabilität, zu längeren und dickeren Wurzeln sowie einem tieferen und stärker differenzierten Wurzelraum. Die höhere ober- und unterirdische Biomasse führt nicht nur zu einer besseren Bodenstabilität, sondern reduziert zudem den Oberflächenabfluss und steigert die (Evapo-) Transpiration. Das vorgestellte Feldexperiment zeigt deutlich, dass die Zugabe eines kommerziellen, den ausgewählten Pflanzen angepassten Mykorrhiza-Inokulums sehr gewinnbringend im ingenieurbiologischen Hangverbau ist und, zumindest für den hier getesteten Anwendungsbereich, unbedingt berücksichtig werden sollte.

The ability of desert plants to adapt to future climate changes and maximize their water-use efficiency will determine their survival. This study uses wood anatomy and δ¹³C and δ¹⁸O isotope analyses to investigate how Moringa peregrina trees in the Egyptian desert have responded to the environment over the last 10 years. Our results show that M. peregrina tree-ring widths (TRWs) have generally declined over the last decade, although individual series are characterized by high variability and low Rbars. Vessel lumen area percentages (VLA%) are low in wet years but increase significantly in dry years, such as the period 2017-2020. Stable δ¹³C isotope values decrease between 2010 (- 23.4‰) and 2020 (- 24.9‰), reflecting an unexpected response to an increase in drought conditions. The mean δ¹⁸O value (± standard error, SE) for the first ten rings of each tree from bark to pith (2020-2010) is 33.0 ‰ ± 0.85 with a range of 29.2-36.3‰, which indicates a common drought signal. The intrinsic water-use efficiency (iWUE) declines gradually with time, from 130.0 µmol mol^-1 in 2010 to 119.4 µmol mol^-1 in 2020. The intercellular carbon concentration (C_i) and C_i/C_a ratio increase over the same period, likely as a result of decreasing iWUE. The results show that M. peregrina trees seem to cool their leaves and the boundary air at the cost of saving water.

Our understanding of wood anatomy and radial growth in tree roots remains very incomplete, particularly with respect to how ecological factors affect root growth at a relatively small spatial scale, i.e., within a single root system. Here, we compared root growth with and without trampling exposure on a hiking trail. We conducted a quantitative analysis of radial growth and wood anatomical changes, including compression wood (CW) and blue rings (BRs), of two adjacent Scots pine roots in high resolution. A total of 32 cross sections from two roots sampled every 25 cm at the same distance from the respective stem were compared. The buried root (B) was completely buried and had an unexposed segment on a hiking trail. In contrast, the exposed root (E) had an exposed segment that was trampled. 1,706 growth rings were analysed for the common period 1954-2015.
We found that the volume of the E root in the trampling zone exceeded ten times the volume of the B root. The root surface area of the exposed sections of the E root was on average 14 times larger than that of the unexposed B root section in the trampling zone. The highest number of missing rings was found in the B root. Root sections sampled at the shortest distance from the stem showed the highest coherence in radial growth pattern, which decreased with increasing distance from the stem.
BRs were recognized for the first time in tree roots. In total 25 tree rings contained BRs, and their occurrence was restricted to cross sections of the exposed root. BRs were formed over the course of 25 calendar years, i.e., in 40% of tree rings from the common period 1954-2015. Mean monthly temperatures for the years with and without BRs formation showed that colder November (p = 0. 012) and, albeit only slightly, colder September (p = 0.051) temperatures favoured formation of BRs in Scots pine roots. In addition, mean monthly precipitation in July (p = 0.017) was significantly higher for BR years, suggesting an impact of moisture availability on the formation of BRs in Scots pine roots. The study highlights a high rate of growth discrepancies within a single root system. Further, altered growth of trampled roots with high proportions of BRs opens a new challenge for future dendroecological studies on tree roots.

Fused Deposition Modeling (FDM), better known as 3D printing, has revolutionized modern manufacturing processes and the ever-increasing use of 3D printers is popular not least because of the wide range of materials available for printing. When applying the FDM process to the development of prototypes, it is possible to go from an idea to a first iteration of the product within a few hours, and from an initial concept to a final product within a few days depending on the complexity of the desired structure. We applied FDM-related open-source 3D software and a 3D printer to produce parts for devices being applied in wood anatomy and dendroecology. In this paper, we present the basic requirements for prototyping by showing detailed examples of new devices developed and produced using a 3D printer and related modeling software.

The rapidly collapsing glacial systems of the Alps produced a large number of melt-water lakes and mires after the Last Glacial Maximum (LGM) in the Late Glacial period. The Rhone-Aare-glacier system gave rise to large moorlands and lakes in the region of the Three Lakes Region of Western Switzerland. When moorlands are formed, they are efficient sinks of atmospheric carbon, but when transformed to agricultural land they are significant C sources. In addition, mires can be used as archives for reconstructing landscape evolution. We explored in more detail the dynamics of the landscape of the Three Lakes Region with a particular focus on the formation and degradation of mires. The Bernese part of the Three Lakes Region developed to become - after the optimisation of the water-levels of the Swiss Jura - the vegetable belt of Switzerland. The situation for agriculture, however, has now become critical due to an overexploitation of the peatland. Until c. 13 ka BP the entire region was hydrologically connected. An additional lake existed at the western end of the plain receiving sediments from the Aare river. Around 13 ka BP, this lake was isolated from the Aare river and completely silted up until c. 10 ka BP when a mire started to form. In the valley floor ('Grosses Moos'), the meandering Aare and the varying level of the nearby lake of Neuchâtel caused a spatio-temporally patchy formation of mires (start of formation: 10-3 ka BP). Strong morphodynamics having high erosion and sedimentation rates and a high variability of the chemical composition of the deposited material prevailed during the early Holocene until c. 7.5 ka BP. The situation remained relatively quiet between 5 and 2 ka BP. However, during the last 2000 years the hydrodynamic and geomorphic activities have increased again. The optimisation of the Swiss Jura water-levels during the nineteenth and twentieth centuries enabled the transformation of moorland into arable land. As a consequence, the moorland strongly degraded. Mean annual C-losses in agricultural land are c. 4.9 t ha^-1 and c. 2.4 t ha^-1 in forests. Because forests limit, but not stop, the degradation of mires, agroforestry might be tested and propagated in future as alternative land-use systems for such sensitive areas.

The formation of annual growth rings is an imperative requirement for studying the dendroecological potential of plant species. Little is known about the distinctness of growth rings in the wood of desert plants in Africa, particularly in southern Mediterranean regions. To fill this knowledge gap, we collected and anatomically analyzed about 300 perennial desert plants from different areas in Egypt. Out of the 300 studied plant species, 94 species were identified as growth-ring forming plants. They belong to 78 genera and 33 families. Trees, shrubs/subshrubs, and perennial or short-lived herbs are represented by 7, 55, and 32 species, respectively. Trees and shrubs represent 7.4% and 58.5% of the total, respectively. The distinctness of growth rings in these species was indicated either by the type of wood porosity, fibers, marginal parenchyma layers or by a mixture of these characteristics. Ring porosity, fiber layer, and parenchyma were the main indicators for growth-ring distinctness in 77.7%, 60.6%, and 33.0% of growth-ring forming plants, respectively. We conclude that some of the desert plants form distinct growth rings depending on site conditions, while precipitation is the main driver for its formation. Further studies on the annual rhythm of xylem formation are required to confirm the annual nature of growth rings and potential dendroecological use of these plants.

Dendrochronology has been applied around the world over decades to reconstruct historical geomorphological events and climatic conditions. Traditionally, this research has been conducted using trees (conifers as well as broad-leaved trees) but, in the last few decades, several shrub and dwarf shrub species have also been shown to be useful for dendrochronological purposes. This study assesses the potential of mountain avens (Dryas octopetala L.) to provide accurately dated information about past debris-flow events. The study site, the Marlt-Graben debris-fan, is located in South Tyrol (Italy). 119 shrubs from three debris-flow tracks were analyzed. The longest radius of each sample was measured and cross-dated to build two chronologies for each debris-flow track, one for each leveé. Correlations between these chronologies and precipitation and temperature data from five climate stations located in the proximity of the study site were calculated. The cross-dating procedure was complex, but a strict grouping of the samples, based on the specific leveés of the tracks, enabled the construction of mean chronologies for each of the individual slopes. Although the development of a mean chronology for the study area was unsuccessful, the cross-dated ages of the single shrubs allowed us to reconstruct debris-flow events by utilizing the minimum age method. The low climate correlations suggest that micro site conditions strongly influence the growth of this dwarf shrub. Although the results of this study suggest that Dryas octopetala may provide useful dendroecological information, additional information about its growth dynamics is required before this potential can be fully realized.

During recent decades, stable oxygen isotopes derived from tree-ring cellulose (δ¹⁸O_TRC) have been frequently utilised as the baseline for palaeoclimatic reconstructions. In this context, numerous studies take advantage of the high sensitivity of trees close to their ecological distribution limit (high elevation or high latitudes). However, this increases the chance that indirect climatic forces such as cold ground induced by permafrost can distort the climate-proxy relationship. In this study, a tree stand of sub-alpine larch trees (Larix decidua Mill.) located in an inner alpine dry valley (Val Bever), Switzerland, was analysed for its δ¹⁸O_TRC variations during the last 180 years. A total of eight L. decidua trees were analysed on an individual base, half of which are located on verified sporadic permafrost lenses approximately 500 m below the expected lower limit of discontinuous permafrost. The derived isotope time series are strongly dependent on variations in summer temperature, precipitation and large-scale circulation patterns (geopotential height fields). The results demonstrate that trees growing outside of the permafrost distribution provide a significantly stronger and more consistent climate-proxy relationship over time than permafrost-affected tree stands. The climate sensitivity of permafrost-affected trees is analogical to the permafrost-free tree stands (positive and negative correlations with temperature and precipitation, respectively) but attenuated partly leading to a complete loss of significance. In particular, decadal summer temperature variations are well reflected in δ¹⁸O_TRC from permafrost-free sites (r = 0.62, p < 0.01), while permafrost-affected sites demonstrate a full lack of this dependency (r = 0.30, p > 0.05). Since both tree stands are located just a few meters away from one another and are subject to the same climatic influences, discrepancies in the isotope time series can only be attributed to variations in the trees' source water that constraints the climatic fingerprints on δ¹⁸O_TRC. If the two individual time series are merged to one local mean chronology, the climatic sensitivity reflects an intermediate between the permafrost-free and –affected δ¹⁸O_TRC time series. It can be deduced, that a significant loss of information on past climate variations arises by simply averaging both tree stands without prior knowledge of differing subsurface conditions.

Moringa peregrina (Forssk.) Fiori, one of 13 species of the Moringaceae family widely distributed throughout the dry tropics, has the potential to become one of the most economically important medicinal plants in Egypt. However, despite its tolerance for drought and heat, it is also threatened by increasing temperatures and decreasing precipitation. Although the phenophase of this species is well documented, almost nothing is known about its period of cambial activity in desert regions. Ring formation and the general environmental adaptability of trees are affected by the timing of cambial activation. In our study site, we observe a distinct coupling of the development of new green leaves at the onset of vegetative growth in October and the phase of cambial activity (November–January). The onset of cambial activity seems to be related to a drop in temperature in October and the onset of torrential rains in the region. There might even be a short phase between the end of cambial activity and the onset of bud formation without xylem formation, but with photosynthetic activity. If so, we assume that all assimilates are stored as non-structural carbohydrates (NSC) in the parenchyma of the new ring. This potential gap opens new questions regarding the correlation between NSC storage capacity and the timing of remobilization for subsequent ring formation.

Non-structural carbohydrates (NSC) reserves are crucial for trees to cope with weather extremes, thus to ensure their survival and ecological plasticity. The NSC reserves can depend on social status, suggesting uneven plasticity of trees at the stand level. In stemwood of Scots pine (Pinus sylvestris L.), which is a widespread and important species, NSC reserves are stored in parenchyma in wood rays (WR). The quantity of WRs is adjusted intra-annually, allowing retrospective analysis of factors affecting their formation. Accordingly, the differences in WR quantity in stemwood of dominant and intermediate (canopy trees with reduced and narrow crowns) maturing Scots pine were assessed by quantitative wood anatomy. Tangential cuts from the outermost 30 tree-rings were analysed. The relative ray area was intermediate, i.e. covering about 5% of the tangential cut, yet expressed high individuality among the trees. The size and amount of WR mainly differed between the earlywood and latewood; WRs in latewood were higher although narrower in comparison to earlywood, yet their total amount was higher in earlywood. Canopy status had only a slight effect, as quantity and height of WR tended to be higher for the intermediate trees, particularly in earlywood. The size and quantity of WR expressed inter-annual variation, which was mainly related to the meteorological conditions prior to the formation of the tree-ring (previous summer and autumn) indicating legacy effects of climatic factors on NSC and susceptibility of trees to cumulative effects of weather extremes. However, the climatic signals in the inter-annual variation of WR were weaker than observed before, likely due to location of the studied stand in the mid-part of the species range. Nevertheless, the observed differences in mean values and inter-annual variation of WR suggested a within-species diversity of carbon allocation patterns, supporting adaptability of the species.

Under changing climate, temporal and spatial stability (stationarity) of growth responses of trees to weather and climate, which has often been presumed without explicit testing, is crucial for prediction of productivity and sustainability of forests. However, considering evolutionary adaptation of tree populations to wide spatiotemporal ecological gradients, extrapolation of linear responses, which could be observed in limited parts of the gradients (certain locality), can result in biased results. Accordingly, the plasticity of responses of tree-ring width of the eastern Baltic populations of Scots pine (Pinus sylvestris L.) to meteorological conditions across the regional climatic gradient was assessed using a mixed generalized additive model. The linear responses were assessed using a bootstrapped correlation analysis for comparison. The radial growth responses of the eastern Baltic population of Scots pine showed explicit regional gradients according to the local climates, indicating gradual shifts in the effects of winter temperature and summer moisture regime. Accordingly, temperature in late-winter and summer, as well as summer water deficit were identified as the main regional drivers of tree-ring width. Their effects were generally non-linear, indicating explicit spatiotemporal gradients in growth responses in the mid-part of species distribution. This also implied limited efficiency of simple linear models for the assessment of radial growth under moderate conditions. The responses to summer temperature indicated local optima, while the responses to drought index showed threshold values. The responses to winter temperature indicated positive effect of warming on growth. In some stands, specific non-stationary responses though were evident, suggesting that trees have adapted (specialized) to certain local climatic conditions, which are changing and thus explaining temporal shifts in growth responses. Nevertheless, the estimated responses suggested phenotypical adaptability limits of the eastern Baltic populations of Scots pine in a longer term, supporting the necessity of climate-smart management for sustainability of forest in the region in the future.

Norway spruce (Picea abies (L.) H. Karst.) is predicted to decrease its abundance in the Eastern Baltic region as a result of climatic changes, and this process is already explicit at the southern limit of species lowland distribution. Still, there are uncertainties about the growth potential of Norway spruce within the region due to the plasticity of local populations. In this regard, an assessment of regional weather-growth responses, assuming a nonlinearity of the ecological relationship, can aid in the clarification of uncertainties regarding growth. Nonlinear regional weather-growth relationships for Norway spruce were assessed based on tree-ring widths from 22 stands spreading from Southern Finland to Northern Germany using dendrochronological methods and a generalized additive mixed model. Temporal and spatial stationarity of local linear weather-growth relationships was evaluated. Considering the drought sensitivity of Norway spruce, meteorological variables related to the summer moisture regime were the main predictors of radial increment, though conditions in winter and spring had complementary effects. Generally, the linear weather-growth relationships were spatially and temporary nonstationary, with some exceptions in Poland and Northern Germany. Explicit local specifics in the linear weather-growth relationships, which are common in the marginal parts of species’ distribution, were observed in Estonia, Latvia, and Poland. The estimated regional weather-growth relationships were mostly nonlinear, implying disproportional responses to climatic changes, particularly to intensifying drought conditions across the studied climatic gradient. Still, the responses to winter temperature suggested that warming might contribute to growth. The estimated linear and nonlinear growth responses indicate strict limitation by drought conditions, implying reductions of increment due to climatic changes southward from Latvia, suggesting the necessity for proactive management. Nevertheless, in the northern part of the analyzed region, the projected climatic changes appear favorable for growth of Norway spruce in the near future.